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The method further includes defining a window, the window defining a second field of view representing a portion of the first field of view such that the second field of view is smaller than the first field of view. Described herein are devices, systems, and methods for processing images captured using SIM to overcome the pre-existing challenges and achieve the benefits as described herein.Īn implementation relates to a method that includes receiving a plurality of images captured using structured illumination microscopy (SIM) in an optical system, each image of the plurality of images having a first field of view. It may be desirable to provide systems and methods for promoting quality control and calibration with imaging optics and associated optical components within a SIM system, particularly a SIM system that is used for imaging biological samples such as nucleotide sequences. In addition to conventional imaging instrument characterization, the SIM imaging optics have additional components that need to be further characterized and validated.

High quality raw images require careful tuning, calibration, and assessment of the optics performance of the imaging instrument. To ensure successful reconstruction, the source raw images should be of high quality. Structured illumination microscopy (SIM) is a class of computational imaging algorithm that reconstructs super resolution images from multiple lower-resolution source images. The subject matter in this section merely represents different approaches, which in and of themselves may also correspond to implementations of the claimed technology. Similarly, a problem mentioned in this section or associated with the subject matter provided as background should not be assumed to have been previously recognized in the prior art. The subject matter discussed in this section should not be assumed to be prior art merely as a result of its mention in this section. 6, 2019, the disclosure of which is incorporated by reference herein, in its entirety. 62/944,687, entitled “Apparatus and Method of Estimating Values from Images,” filed Dec.